lamp-2a sirna Search Results


sirna against human lamp2a  (Hayashi Kasei Co)
90
Hayashi Kasei Co sirna against human lamp2a
( A ) Representative fluorescent images of GAPDH-HT 21 h after labeling with TMR-HT ligand in HeLa cells transfected with <t>nontargeting-siRNA</t> (left) or LAMP2A-siRNA (right). Bar = 20 µm. ( B,C ) Quantitative analyses of GAPDH-HT lysosomal translocation in cells transfected with nontargeting (Non)- and LAMP2A-siRNA. ( B ) Cells having more than 5 dots of GAPDH-HT were classified as GAPDH-HT dot-positive cells. We counted the number of GAPDH-HT dot-positive cells in 50–70 GAPDH-HT-expressing cells. ( C ) We assessed the number of GAPDH-HT dots per cell. The percentage of GAPDH-HT dot-positive cells and the number of GADPH-HT dots per cell were significantly decreased by siRNA-mediated LAMP2A-knockdown. ** p<0.001 vs cells treated with nontargeting-siRNA (unpaired t -test, n = 16 in B , n = 57 for nontargeting-siRNA and n = 74 for LAMP2A-siRNA in C ). ( D ) Representative fluorescent images of GAPDH-HT taken 21 h after labeling with the TMR-HT ligand in HeLa cells treated with vehicle (0.1% DMSO, 0.1% methanol, left upper), serum free medium (0.1% DMSO, 0.1% methanol, center upper), H 2 O 2 (100 µM, right upper), mycophenolic acid (MPA; 10 µM, left lower), SB202190 (20 µM, left center) or cycloheximide (CHX; 20 µg/ml, right lower). Bar = 20 µm. ( E,F ) Quantitative analyses of GAPDH-HT lysosomal translocation in cells treated with CMA activators or inhibitors the percentages of GAPDH-HT dot-positive cells ( E ) and the number of GAPDH-HT dots per cell ( F ). Percentages of GAPDH-HT dot-positive cells and the numbers of GAPDH-HT dots per cell were significantly increased by CMA activators (serum free medium, H 2 O 2 and MPA), while they were significantly decreased by CMA inhibitors (SB202190 and CHX). * p<0.01, ** p<0.001 vs cells treated with vehicle (unpaired t -test, n = 12 for cells treated with vehicle, n = 8 for cells treated with CMA activators and inhibitors in E , n = 96 for vehicle, n = 61 for serum free, n = 45 for H 2 O 2 , n = 58 for MPA, n = 52 for SM202190 and n = 41 for CHX in F ).
Sirna Against Human Lamp2a, supplied by Hayashi Kasei Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lamp-2a sirna  (Eurofins)
90
Eurofins lamp-2a sirna
( A ) Representative fluorescent images of GAPDH-HT 21 h after labeling with TMR-HT ligand in HeLa cells transfected with <t>nontargeting-siRNA</t> (left) or LAMP2A-siRNA (right). Bar = 20 µm. ( B,C ) Quantitative analyses of GAPDH-HT lysosomal translocation in cells transfected with nontargeting (Non)- and LAMP2A-siRNA. ( B ) Cells having more than 5 dots of GAPDH-HT were classified as GAPDH-HT dot-positive cells. We counted the number of GAPDH-HT dot-positive cells in 50–70 GAPDH-HT-expressing cells. ( C ) We assessed the number of GAPDH-HT dots per cell. The percentage of GAPDH-HT dot-positive cells and the number of GADPH-HT dots per cell were significantly decreased by siRNA-mediated LAMP2A-knockdown. ** p<0.001 vs cells treated with nontargeting-siRNA (unpaired t -test, n = 16 in B , n = 57 for nontargeting-siRNA and n = 74 for LAMP2A-siRNA in C ). ( D ) Representative fluorescent images of GAPDH-HT taken 21 h after labeling with the TMR-HT ligand in HeLa cells treated with vehicle (0.1% DMSO, 0.1% methanol, left upper), serum free medium (0.1% DMSO, 0.1% methanol, center upper), H 2 O 2 (100 µM, right upper), mycophenolic acid (MPA; 10 µM, left lower), SB202190 (20 µM, left center) or cycloheximide (CHX; 20 µg/ml, right lower). Bar = 20 µm. ( E,F ) Quantitative analyses of GAPDH-HT lysosomal translocation in cells treated with CMA activators or inhibitors the percentages of GAPDH-HT dot-positive cells ( E ) and the number of GAPDH-HT dots per cell ( F ). Percentages of GAPDH-HT dot-positive cells and the numbers of GAPDH-HT dots per cell were significantly increased by CMA activators (serum free medium, H 2 O 2 and MPA), while they were significantly decreased by CMA inhibitors (SB202190 and CHX). * p<0.01, ** p<0.001 vs cells treated with vehicle (unpaired t -test, n = 12 for cells treated with vehicle, n = 8 for cells treated with CMA activators and inhibitors in E , n = 96 for vehicle, n = 61 for serum free, n = 45 for H 2 O 2 , n = 58 for MPA, n = 52 for SM202190 and n = 41 for CHX in F ).
Lamp 2a Sirna, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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sirna for lamp2a silamp2a  (Bioneer Corporation)
90
Bioneer Corporation sirna for lamp2a silamp2a
( A ) Representative fluorescent images of GAPDH-HT 21 h after labeling with TMR-HT ligand in HeLa cells transfected with <t>nontargeting-siRNA</t> (left) or LAMP2A-siRNA (right). Bar = 20 µm. ( B,C ) Quantitative analyses of GAPDH-HT lysosomal translocation in cells transfected with nontargeting (Non)- and LAMP2A-siRNA. ( B ) Cells having more than 5 dots of GAPDH-HT were classified as GAPDH-HT dot-positive cells. We counted the number of GAPDH-HT dot-positive cells in 50–70 GAPDH-HT-expressing cells. ( C ) We assessed the number of GAPDH-HT dots per cell. The percentage of GAPDH-HT dot-positive cells and the number of GADPH-HT dots per cell were significantly decreased by siRNA-mediated LAMP2A-knockdown. ** p<0.001 vs cells treated with nontargeting-siRNA (unpaired t -test, n = 16 in B , n = 57 for nontargeting-siRNA and n = 74 for LAMP2A-siRNA in C ). ( D ) Representative fluorescent images of GAPDH-HT taken 21 h after labeling with the TMR-HT ligand in HeLa cells treated with vehicle (0.1% DMSO, 0.1% methanol, left upper), serum free medium (0.1% DMSO, 0.1% methanol, center upper), H 2 O 2 (100 µM, right upper), mycophenolic acid (MPA; 10 µM, left lower), SB202190 (20 µM, left center) or cycloheximide (CHX; 20 µg/ml, right lower). Bar = 20 µm. ( E,F ) Quantitative analyses of GAPDH-HT lysosomal translocation in cells treated with CMA activators or inhibitors the percentages of GAPDH-HT dot-positive cells ( E ) and the number of GAPDH-HT dots per cell ( F ). Percentages of GAPDH-HT dot-positive cells and the numbers of GAPDH-HT dots per cell were significantly increased by CMA activators (serum free medium, H 2 O 2 and MPA), while they were significantly decreased by CMA inhibitors (SB202190 and CHX). * p<0.01, ** p<0.001 vs cells treated with vehicle (unpaired t -test, n = 12 for cells treated with vehicle, n = 8 for cells treated with CMA activators and inhibitors in E , n = 96 for vehicle, n = 61 for serum free, n = 45 for H 2 O 2 , n = 58 for MPA, n = 52 for SM202190 and n = 41 for CHX in F ).
Sirna For Lamp2a Silamp2a, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirna for lamp2a silamp2a/product/Bioneer Corporation
Average 90 stars, based on 1 article reviews
sirna for lamp2a silamp2a - by Bioz Stars, 2026-03
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lamp2a sirna duplex  (Ambio Inc)
90
Ambio Inc lamp2a sirna duplex
( A ) Representative fluorescent images of GAPDH-HT 21 h after labeling with TMR-HT ligand in HeLa cells transfected with <t>nontargeting-siRNA</t> (left) or LAMP2A-siRNA (right). Bar = 20 µm. ( B,C ) Quantitative analyses of GAPDH-HT lysosomal translocation in cells transfected with nontargeting (Non)- and LAMP2A-siRNA. ( B ) Cells having more than 5 dots of GAPDH-HT were classified as GAPDH-HT dot-positive cells. We counted the number of GAPDH-HT dot-positive cells in 50–70 GAPDH-HT-expressing cells. ( C ) We assessed the number of GAPDH-HT dots per cell. The percentage of GAPDH-HT dot-positive cells and the number of GADPH-HT dots per cell were significantly decreased by siRNA-mediated LAMP2A-knockdown. ** p<0.001 vs cells treated with nontargeting-siRNA (unpaired t -test, n = 16 in B , n = 57 for nontargeting-siRNA and n = 74 for LAMP2A-siRNA in C ). ( D ) Representative fluorescent images of GAPDH-HT taken 21 h after labeling with the TMR-HT ligand in HeLa cells treated with vehicle (0.1% DMSO, 0.1% methanol, left upper), serum free medium (0.1% DMSO, 0.1% methanol, center upper), H 2 O 2 (100 µM, right upper), mycophenolic acid (MPA; 10 µM, left lower), SB202190 (20 µM, left center) or cycloheximide (CHX; 20 µg/ml, right lower). Bar = 20 µm. ( E,F ) Quantitative analyses of GAPDH-HT lysosomal translocation in cells treated with CMA activators or inhibitors the percentages of GAPDH-HT dot-positive cells ( E ) and the number of GAPDH-HT dots per cell ( F ). Percentages of GAPDH-HT dot-positive cells and the numbers of GAPDH-HT dots per cell were significantly increased by CMA activators (serum free medium, H 2 O 2 and MPA), while they were significantly decreased by CMA inhibitors (SB202190 and CHX). * p<0.01, ** p<0.001 vs cells treated with vehicle (unpaired t -test, n = 12 for cells treated with vehicle, n = 8 for cells treated with CMA activators and inhibitors in E , n = 96 for vehicle, n = 61 for serum free, n = 45 for H 2 O 2 , n = 58 for MPA, n = 52 for SM202190 and n = 41 for CHX in F ).
Lamp2a Sirna Duplex, supplied by Ambio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirna-targeting lamp2a mrna  (Sangon Biotech)
90
Sangon Biotech sirna-targeting lamp2a mrna
( A ) Representative fluorescent images of GAPDH-HT 21 h after labeling with TMR-HT ligand in HeLa cells transfected with <t>nontargeting-siRNA</t> (left) or LAMP2A-siRNA (right). Bar = 20 µm. ( B,C ) Quantitative analyses of GAPDH-HT lysosomal translocation in cells transfected with nontargeting (Non)- and LAMP2A-siRNA. ( B ) Cells having more than 5 dots of GAPDH-HT were classified as GAPDH-HT dot-positive cells. We counted the number of GAPDH-HT dot-positive cells in 50–70 GAPDH-HT-expressing cells. ( C ) We assessed the number of GAPDH-HT dots per cell. The percentage of GAPDH-HT dot-positive cells and the number of GADPH-HT dots per cell were significantly decreased by siRNA-mediated LAMP2A-knockdown. ** p<0.001 vs cells treated with nontargeting-siRNA (unpaired t -test, n = 16 in B , n = 57 for nontargeting-siRNA and n = 74 for LAMP2A-siRNA in C ). ( D ) Representative fluorescent images of GAPDH-HT taken 21 h after labeling with the TMR-HT ligand in HeLa cells treated with vehicle (0.1% DMSO, 0.1% methanol, left upper), serum free medium (0.1% DMSO, 0.1% methanol, center upper), H 2 O 2 (100 µM, right upper), mycophenolic acid (MPA; 10 µM, left lower), SB202190 (20 µM, left center) or cycloheximide (CHX; 20 µg/ml, right lower). Bar = 20 µm. ( E,F ) Quantitative analyses of GAPDH-HT lysosomal translocation in cells treated with CMA activators or inhibitors the percentages of GAPDH-HT dot-positive cells ( E ) and the number of GAPDH-HT dots per cell ( F ). Percentages of GAPDH-HT dot-positive cells and the numbers of GAPDH-HT dots per cell were significantly increased by CMA activators (serum free medium, H 2 O 2 and MPA), while they were significantly decreased by CMA inhibitors (SB202190 and CHX). * p<0.01, ** p<0.001 vs cells treated with vehicle (unpaired t -test, n = 12 for cells treated with vehicle, n = 8 for cells treated with CMA activators and inhibitors in E , n = 96 for vehicle, n = 61 for serum free, n = 45 for H 2 O 2 , n = 58 for MPA, n = 52 for SM202190 and n = 41 for CHX in F ).
Sirna Targeting Lamp2a Mrna, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirna-targeting lamp2a mrna/product/Sangon Biotech
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Image Search Results


( A ) Representative fluorescent images of GAPDH-HT 21 h after labeling with TMR-HT ligand in HeLa cells transfected with nontargeting-siRNA (left) or LAMP2A-siRNA (right). Bar = 20 µm. ( B,C ) Quantitative analyses of GAPDH-HT lysosomal translocation in cells transfected with nontargeting (Non)- and LAMP2A-siRNA. ( B ) Cells having more than 5 dots of GAPDH-HT were classified as GAPDH-HT dot-positive cells. We counted the number of GAPDH-HT dot-positive cells in 50–70 GAPDH-HT-expressing cells. ( C ) We assessed the number of GAPDH-HT dots per cell. The percentage of GAPDH-HT dot-positive cells and the number of GADPH-HT dots per cell were significantly decreased by siRNA-mediated LAMP2A-knockdown. ** p<0.001 vs cells treated with nontargeting-siRNA (unpaired t -test, n = 16 in B , n = 57 for nontargeting-siRNA and n = 74 for LAMP2A-siRNA in C ). ( D ) Representative fluorescent images of GAPDH-HT taken 21 h after labeling with the TMR-HT ligand in HeLa cells treated with vehicle (0.1% DMSO, 0.1% methanol, left upper), serum free medium (0.1% DMSO, 0.1% methanol, center upper), H 2 O 2 (100 µM, right upper), mycophenolic acid (MPA; 10 µM, left lower), SB202190 (20 µM, left center) or cycloheximide (CHX; 20 µg/ml, right lower). Bar = 20 µm. ( E,F ) Quantitative analyses of GAPDH-HT lysosomal translocation in cells treated with CMA activators or inhibitors the percentages of GAPDH-HT dot-positive cells ( E ) and the number of GAPDH-HT dots per cell ( F ). Percentages of GAPDH-HT dot-positive cells and the numbers of GAPDH-HT dots per cell were significantly increased by CMA activators (serum free medium, H 2 O 2 and MPA), while they were significantly decreased by CMA inhibitors (SB202190 and CHX). * p<0.01, ** p<0.001 vs cells treated with vehicle (unpaired t -test, n = 12 for cells treated with vehicle, n = 8 for cells treated with CMA activators and inhibitors in E , n = 96 for vehicle, n = 61 for serum free, n = 45 for H 2 O 2 , n = 58 for MPA, n = 52 for SM202190 and n = 41 for CHX in F ).

Journal: PLoS ONE

Article Title: Establishment of a Novel Fluorescence-Based Method to Evaluate Chaperone-Mediated Autophagy in a Single Neuron

doi: 10.1371/journal.pone.0031232

Figure Lengend Snippet: ( A ) Representative fluorescent images of GAPDH-HT 21 h after labeling with TMR-HT ligand in HeLa cells transfected with nontargeting-siRNA (left) or LAMP2A-siRNA (right). Bar = 20 µm. ( B,C ) Quantitative analyses of GAPDH-HT lysosomal translocation in cells transfected with nontargeting (Non)- and LAMP2A-siRNA. ( B ) Cells having more than 5 dots of GAPDH-HT were classified as GAPDH-HT dot-positive cells. We counted the number of GAPDH-HT dot-positive cells in 50–70 GAPDH-HT-expressing cells. ( C ) We assessed the number of GAPDH-HT dots per cell. The percentage of GAPDH-HT dot-positive cells and the number of GADPH-HT dots per cell were significantly decreased by siRNA-mediated LAMP2A-knockdown. ** p<0.001 vs cells treated with nontargeting-siRNA (unpaired t -test, n = 16 in B , n = 57 for nontargeting-siRNA and n = 74 for LAMP2A-siRNA in C ). ( D ) Representative fluorescent images of GAPDH-HT taken 21 h after labeling with the TMR-HT ligand in HeLa cells treated with vehicle (0.1% DMSO, 0.1% methanol, left upper), serum free medium (0.1% DMSO, 0.1% methanol, center upper), H 2 O 2 (100 µM, right upper), mycophenolic acid (MPA; 10 µM, left lower), SB202190 (20 µM, left center) or cycloheximide (CHX; 20 µg/ml, right lower). Bar = 20 µm. ( E,F ) Quantitative analyses of GAPDH-HT lysosomal translocation in cells treated with CMA activators or inhibitors the percentages of GAPDH-HT dot-positive cells ( E ) and the number of GAPDH-HT dots per cell ( F ). Percentages of GAPDH-HT dot-positive cells and the numbers of GAPDH-HT dots per cell were significantly increased by CMA activators (serum free medium, H 2 O 2 and MPA), while they were significantly decreased by CMA inhibitors (SB202190 and CHX). * p<0.01, ** p<0.001 vs cells treated with vehicle (unpaired t -test, n = 12 for cells treated with vehicle, n = 8 for cells treated with CMA activators and inhibitors in E , n = 96 for vehicle, n = 61 for serum free, n = 45 for H 2 O 2 , n = 58 for MPA, n = 52 for SM202190 and n = 41 for CHX in F ).

Article Snippet: Nontargeting-siRNA was used as a negative control of siRNA transfection. siRNAs against human LAMP2A (sense: 5′-GGCAGGAGUACUUAUUCUAGU-3′ , antisense: 5′-UAGAAUAAGUACUCCUGCCAA-3′ ) and human Atg5 (sense: 5′-CACUUUCAGAAGGUUAUGAGA-3′ , antisense: 5′-UCAUAACCUUCUGAAAGUGCU-3′ ) were constructed by Hayashi-kasei (Osaka, Japan). siRNA (50 pmol) was transfected to HeLa cells 1 day after cell spread using Lipofectamine RNAiMAX.

Techniques: Labeling, Transfection, Translocation Assay, Expressing, Knockdown